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E0276

Sigma-Aldrich

EPPS

BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)

Sinónimos:

4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), HEPPS

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About This Item

Fórmula empírica (notación de Hill):
C9H20N2O4S
Número de CAS:
16052-06-5
Peso molecular:
252.33
Beilstein/REAXYS Number:
3957385
EC Number:
240-198-8
MDL number:
MFCD00006160
UNSPSC Code:
12161700
PubChem Substance ID:
24894366
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

400

assay

≥99.5% (titration)

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and Total Aerobic Microbial Count, tested

pH

7.3-8.7

useful pH range

7.3-8.7

pKa (25 °C)

8.0

pKa 

8

mp

237-239 °C (lit.)

solubility

H2O: 25 g + 50 mL, clear, colorless

cation traces

heavy metals (as Pb): ≤5 ppm

λmax

A290 at ≤0.1 (C=33% w/w)

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCCS(O)(=O)=O)CC1

InChI

1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)

InChI key

OWXMKDGYPWMGEB-UHFFFAOYSA-N

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General description

EPPS or 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (HEPPS) is a zwitterionic buffer substance from the group of good buffers. It effectively maintains constant pH in a solution, which is necessary for many biological and biochemical reactions. EPPS buffer is widely used in cell culture experiments and biological research.

Application

EPPS may be used as a buffer solution to stabilize the pH in sea water samples during the titration experiments.

Features and Benefits

  • BioPerformance Certified, high-quality level (400), ensures reliable and consistent results.
  • Suitable for mammalian cell culture techniques

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SLCH3016
SLCC9508
SLCC0971
SLBV5843
SLBR1133V

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lonization Enthalpies of Some Common Zvvitterionic Hydrogen-lon Buffers (HEPES, PIPES, HEPPS and BES) for Biological Research
Roig T, et al.
Journal of Separation Science, 993(47), 899-90j-899-90j (1993)
Feng Xie et al.
Cancer letters, 364(2), 106-117 (2015-05-17)
Cervical cancer is often associated with eosinophil (EOS) infiltration, but the source and the role of EOS are still largely unknown. Our previous work has established that thymic stromal lymphopoietin (TSLP) can stimulate the growth of cervical cancer cell in
Xiannu Jin et al.
Journal of pharmacological and toxicological methods, 70(2), 188-194 (2014-08-26)
Malaria is a major health concern and affects over 300million people a year. Accordingly, there is an urgent need for new efficacious anti-malarial drugs. A major challenge in developing new anti-malarial drugs is to design active molecules that have preferable
Calvin R Justus et al.
Experimental cell research, 334(1), 100-113 (2015-04-08)
The effect of acidosis, a biochemical hallmark of the tumor microenvironment, on cancer progression and metastasis is complex. Both pro- and anti-tumorigenic effects of acidosis have been reported and the acidic microenvironment has been exploited for specific delivery of drugs
Anindya Sarkar et al.
The Journal of biological chemistry, 290(35), 21615-21628 (2015-07-03)
Heat shock protein 90 (hsp90) drives heme insertion into the β1 subunit of soluble guanylate cyclase (sGC) β1, which enables it to associate with a partner sGCα1 subunit and mature into a nitric oxide (NO)-responsive active form. We utilized fluorescence

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