EMT6/MTXR

96042322, mouse unknown/unspecified, Epithelial-like

• UNSPSC Code: 41106514

Propiedades

• product name: EMT6/MTXR, 96042322
• biological source: mouse unknown/unspecified
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse mammary, drug-resistant

Cell Line Description

The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.

Application

Drug-resistance studies

Culture Medium

MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml methotrexate + 15% Foetal Bovine Serum (FBS).. Resuscitate in drug-free medium; add drug once growth is fully established

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA;5% CO₂; 37ºC

Other Notes

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