NP cl.25

95051114, Reactivity: Anti-Measles Virus Nucleoprotein

• UNSPSC Code: 41106514

Propiedades

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 95051114-DNA-5UG; pkg of vial of cells 95051114-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: Sp2/0 Ag-14
• Reactivity: Anti-Measles Virus Nucleoprotein
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse Sp2/0 x Mouse BALB/c spleen

Cell Line Description

NP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.

Immunogen

Measles virus (strain Hallè)

Application

Diagnosis, study of measles virus infection

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 1-5x100,000 cells/ml; 5% CO₂; 37°C

Other Notes

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