BRL-1

94121649, rat liver, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: BRL-1, 94121649
• biological source: rat liver
• growth mode: Adherent
• karyotype: Diploid or pseudodiploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Rat epithelial cells

Cell Line Description

Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.

Application

Feeder cells for co-culture with freshly isolated hepatocytes.

Culture Medium

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Subculture Routine

Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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