AT6.1

94101452, rat prostate, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: AT6.1, 94101452
• biological source: rat prostate
• growth mode: Adherent
• karyotype: 44XY, +4, + 12 del(3)(3q32-36)del(15)p14
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Rat dorsal prostatic adenocarcinoma

Cell Line Description

A highly metastatic, anaplastic, androgen-independent sub-line which spontaneously developed during the serial passage of the non-metastatic, well-differentiated, androgen-responsive R-3327-H sub-lines in the laboratory of Dr J Schalken, Nijmegan, The Netherlands.

Application

Used in studies in which micro-cell mediated chromosomal transfer of human chromosome 11 suppressed metastatic ability. This suppresser function was localised to 11p11.2-13.

Culture Medium

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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