EZZ (TOU II-4)

93093003, human blood, Lymphoblast

• UNSPSC Code: 41106514

Propiedades

• product name: EZZ (TOU II-4), 93093003
• biological source: human blood
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human B cell lymphocyte, EBV transformed

Cell Line Description

Peripheral blood lymphocytes from a male Tunisian, immortalised by EBV transformation. The cell line contains the following deletions, at the homozygous state, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion I (del G1-EP1-A1-GP-G2-G4) Gm-Am genotype Gm b; -; -A2m2/Gm b ; -; -A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). The EZZ deletion (deletion I), which encompasses 160 kb involves two highly homologous regions located 13 kb downstream of IGHG3 and 13 kb downstream of IGHG4.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures at 5-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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