NCTC 4093

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Propiedades

• biological source: mouse embryo
• packaging: tube of 5 μg 90102530-DNA-5UG; pkg of vial of cells 90102530-1VL
• growth mode: Adherent
• karyotype: Modal no. 71-73; hypotetraploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse C57B1/KaLw embryo

Cell Line Description

Derived by cloning from a parent line produced from normal mouse embryo tissue (C57B1/KaLw). The cells have spontaneously transformed in vitro. The cells are negative for polyoma virus infection but are susceptible to the virus.

Application

Transformation and virus studies: Polyoma virus

Culture Medium

NCTC 135 100% + 2mM Glutamine.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using mechanical methods to dislodge the cells (e.g. scraping); 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.