K562 Cell line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89121407, human blood (chronic myelogenous leukemia), Lymphoblast

• Sinónimos: K-562 Cells
• UNSPSC Code: 41106514

Propiedades

• biological source: human blood (chronic myelogenous leukemia)
• packaging: tube of 5 μg 89121407-DNA-5UG; pkg of vial of cells 89121407-1VL
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian chronic myelogenous leukaemia

Cell Line Description

Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis. Population highly undifferentiated and of the granulocytic series. Can be used as a highly sensitive target for the in vitro natural killer assay. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series.

Application

K562 cell line has been used to study the protecting role of cluster of differentiation-39 (CD39) attenuation against DNA damage. It has also been used to study the relevance of a novel sorting approach for separating cells, called “Slice and Dice Sorting”.

Natural killer assay and tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 8
D16S539: 11,12
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8,9
vWA: 16

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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