GH1

89072610, rat brain, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: GH1, 89072610
• biological source: rat brain
• growth mode: Semi-adherent
• karyotype: Hypodiploid, modal no. 40.
• morphology: Epithelial
• products: Rat growth hormone, lactogenic hormones.
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Rat Wistar-Furth pituitary tumour

Cell Line Description

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

Application

Hormone studies

Culture Medium

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

Other Notes

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