TM4

88111401, mouse testis, Not specified

• UNSPSC Code: 41106514

Propiedades

• product name: TM4, 88111401
• biological source: mouse testis
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Transferrin, H-Y Ag, novel retinol-binding protein
• receptors: Androgen, oestrogen and progesterone receptors
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse BALB/c testis sertoli cell

Cell Line Description

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

Application

Hormone studies

Culture Medium

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

Other Notes

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