COLO 320DM

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87061205, human colon, Round & Refractile

• UNSPSC Code: 41106514

Propiedades

• biological source: human colon
• packaging: tube of 5 μg 87061205-DNA-5UG; pkg of vial of cells 87061205-1VL
• growth mode: Suspension
• karyotype: Hyperdiploid, modal no. 53
• morphology: Round & Refractile
• products: Serotonin, epinephrine, NPP, Adrenocorticotropic hormone (ACTH) and PTH
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Isolated from a moderately undifferentiated adenocarcinoma from the sigmoid colon of a 55 year old Caucasian female. The cells have double minute (DM) chromosomes.

Application

Hormone studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 8,9
TPOX: 8,9
vWA: 15,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. may semi-adhere to culture flask.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.