3T3 Swiss Albino

85022108, mouse embryo, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: 3T3 Swiss Albino, 85022108
• biological source: mouse embryo
• growth mode: Adherent
• karyotype: 2n = 40; hypertriploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

General description

Product has lost contact inhibition

Cell Line Origin

Mouse Swiss Albino embryo fibroblast

Cell Line Description

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

Application

Contact inhibition and virus studies: polyoma and SV40 viruses

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.