WEHI-231

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85022107, mouse blood, Lymphoblast

• UNSPSC Code: 41106514

Propiedades

• biological source: mouse blood
• packaging: tube of 5 μg 85022107-DNA-5UG; pkg of vial of cells 85022107-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Surface Immunoglobulin M (IgM)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse B cell lymphoma

Cell Line Description

Expresses surface IgM but does not secrete Ig. Secretion of IgM can be induced with LPS. WEHI 231 is a B cell lymphoma of BALB/c x NXB F1 origin induced by mineral oil injection.

Application

Tumourigenicity studies

Culture Medium

DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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