Bu25 TK-

85022104, human cervix, Epithelial

• UNSPSC Code: 41106514

Propiedades

• Nombre del producto: Bu25 TK-, 85022104
• biological source: human cervix
• packaging: tube of 5 μg 85022104-DNA-5UG; pkg of vial of cells 85022104-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human cervical carcinoma

Cell Line Description

HeLa derivative rendered TK- by selection in BUdR.

Application

Transfection studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA. 5% CO₂; 37°C. Passage every 2-3 days when confluent at 1:3 dilution.

Other Notes

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