P3X63Ag8

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Propiedades

• biological source: mouse skin
• packaging: tube of 5 μg 85011401-DNA-5UG; pkg of vial of cells 85011401-1VL
• growth mode: Suspension
• karyotype: Aneuploid
• morphology: Lymphoblast
• products: Immunoglobulin G (IgG1) (kappa)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse myeloma

Cell Line Description

Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20μg/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.

Application

Fusion partner

Culture Medium

DMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 5x10,000 to 3x100,000 cells/ml; 5% CO₂; 37°C. Cells will achieve maximum cell density of 5x100,000 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.