OX-115

13082229, from Rat x Rat Hybridoma

• UNSPSC Code: 41106514

Propiedades

• biological source: Rat x Rat Hybridoma
• ImmunoDon: Rat DA spleen
• Myeloma: Y3
• Reactivity: Recombinant CD147 domain-0
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Description

The hybridoma cell line OX-115 recognises an alternatively spliced domain of mouse CD147.

Immunogen

Recombinant CD147

Application

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.