h1RPE7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Propiedades

• biological source: human eye
• packaging: tube of 5 μg 09061602-DNA-5UG; pkg of vial of cells 09061602-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Polygonal
• products: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Descripción

Cell Line Origin

An immortalized retinal pigment epithelial cell line

Cell Line Description

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

Culture Medium

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.