HeLa (AC-free)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Propiedades

• biological source: human cervix
• packaging: tube of 5 μg 08011102-DNA-5UG; pkg of vial of cells 08011102-1VL
• growth mode: Aggregates in suspension
• karyotype: 2n = 46, aneuploid
• morphology: Spherical
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human cervical carcinoma, serum-free, animal component (AC) free

Cell Line Description

HeLa cell line (Sigma catalogue no. 93021013) adapted to grow in animal component free medium (EX-CELL HeLa Serum-Free Medium for HeLa Cells Sigma cat no 14591C).

Application

Virus studies

Culture Medium

EX-CELL HeLa Serum-Free Medium (Sigma cat no 14591C)+ 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Subculture Routine

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.