T7

7021402, mouse lung, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: T7, 07021402
• biological source: mouse lung
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse Type-II lung epithelial cell

Cell Line Description

Fully differentiated Type-II alveolar epithelial cell line derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33 - 35°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

Culture Medium

DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 additional supplements - please see ′Additional Literature Report′ field below for full details. ECACC can supply an information sheet on the preparation of the supplements on request - contact ECAC

Subculture Routine

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.