B95a

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA, 1092505, cotton-top tamarin monkey blood, Not specified

• UNSPSC Code: 41106514

Propiedades

• biological source: cotton-top tamarin monkey blood
• description: Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• packaging: tube of 5 μg 01092505-DNA-5UG; pkg of vial of cells 01092505-1VL
• growth mode: Semi-adherent
• karyotype: Not specified
• morphology: Not specified
• receptors: Not specified

Descripción

Cell Line Origin

B-lymphoblastoid, EBV-transformed

Cell Line Description

B95a is a derivative of the cell line B95-8 and was adapted to growth in adherence to the substrate surface. The parent line B95-8 was derived from a cotton-top tamarin (Saguinas oedipus). The B-lymphoblastoid cells are transformed with Epstein-Barr virus (EBV). B95a has been reported to be 10,000-fold more sensitive to measles virus present in clinical specimens than Vero cells and are useful host cells for the isolation of measles virus.

Packaging

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA.

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442) (for growth medium) or 2% FBS / FCS (F2442) (for maintenance medium).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 every 3-4 days using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C; cells grow initially as a monolayer, but will pile up and detach in maintenance medium.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.