C7438

Chaperonin 10 from Escherichia coli

≥95.0% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

• Sinónimos: GroES
• MDL number: MFCD00163256
• UNSPSC Code: 12352200
• NACRES: NA.32

Propiedades

• biological source: Escherichia coli
• Quality Level: 200
• recombinant: expressed in E. coli overproducing strain
• assay: ≥95.0% (SDS-PAGE)
• form: lyophilized powder
• technique(s): activity assay: suitable
• UniProt accession no.: P61604
• storage temp.: 2-8°C
• Gene Information: human ... HSPE1(3336)

Descripción

Biochem/physiol Actions

Chaperonin60 (GroEL) and chaperonin10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.

Packaging

Package size based on protein content.

Physical form

Lyophilized powder containing Tris buffer salts, potassium chloride, dithiothreitol and trehalose as stabilizer.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)