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Key Documents

C-28014

Sigma-Aldrich

Mesenchymal Stem Cell Chondrogenic Differentiation Medium

Ready-to use kit including Basal Medium and SupplementMix (without Inducers), 100 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

packaging

pkg of 100 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

General description

Mesenchymal Stem Cell Chondrogenic Differentiation Medium w/o Inducers (Ready-to-use)

Application

Mesenchymal Stem Cell Chondrogenic Differentiation Medium is a defined and serum-free system for the directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue into chondrogenic lineages.Recommended for:Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)Note: this MSC Chondrogenic Differentiation Medium w/o Inducers must be supplemented with adequate chondrogenic inducers by the customer.

Quality

All lots of PromoCell Mesenchymal Stem Cell Media are subjected to comprehensive quality control tests using primary human mesenchymal stem cells. Each lot of MSC Differentiation Media is tested for the capacity to induce directed differentiation into the respective lineages in MSC. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

Storage Class

10 - Combustible liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Mesenchymal Stem Cell Characterization

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

Neuronal Mesenchymal Stem Cells

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocolos

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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