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MilliporeSigma
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C-27115

Sigma-Aldrich

Chondrocyte Growth Medium

Basal Medium, phenol red-free, 500 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

solution

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Chondrocyte Basal Medium, phenol red-free (supplements sold separately)

Application

Chondrocyte Growth Medium has been developed for the in vitro cultivation of human chondrocytes. The fetal calf serum in the medium is strictly pretested for optimal performance. The medium is optimized for primary human cells, but can also be used for primary porcine and rat chondrocytes.Recommended for:Human Chondrocytes (HCH)

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Chondrocyte Growth Medium are subjected to comprehensive quality control tests using primary human chondrocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested chondrocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Subculture Routine

Click here for more information.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Artículos

Primary Human Chondrocytes and Chondrocyte Growth Media

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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