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Key Documents

C265

Sigma-Aldrich

Monoclonal Anti-CaM Kinase IIα (CaMKIIα) antibody produced in mouse

clone 6G9, purified immunoglobulin, buffered aqueous solution

Sinónimos:

Anti-CAMKA, Anti-CaMKIINalpha, Anti-CaMKIIalpha, Anti-MRD53, Anti-MRT63

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About This Item

MDL number:
MFCD02095961
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6G9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, mouse

technique(s)

indirect immunofluorescence: suitable using rat hippocampus
western blot: 0.5-1.0 μg/mL

isotype

IgG1

UniProt accession no.

P11275

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Camk2a(25400)

General description

CaM Kinase II α is a Ca2+/calmodulin-dependent kinase that belongs to the serine/threonine protein kinases family. It facilitates the regulation of NMDAR-dependent AMPA receptor trafficking to the synapses. Mouse anti-CaM kinase II α antibody reacts specifically with phosphorylated and non-phosphorylated forms of native and recombinant CaM (Ca2+/calmodulin-dependent) kinase II α subunit in rats. The product has shown no reactivity for phosphorylated or non-phosphorylated β subunit (60kD).

Immunogen

partially purified rat CaM kinase II.

Application

Monoclonal Anti-CaM Kinase IIα (CaMKIIα) antibody produced in mouse has been used in immunohistochemistry and western blotting.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Naohiro Kon et al.
Genes & development, 28(10), 1101-1110 (2014-05-17)
Daily behavioral rhythms in mammals are governed by the central circadian clock, located in the suprachiasmatic nucleus (SCN). The behavioral rhythms persist even in constant darkness, with a stable activity time due to coupling between two oscillators that determine the
Joana S Ferreira et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(22), 8462-8479 (2015-06-05)
NMDA receptors play a central role in shaping the strength of synaptic connections throughout development and in mediating synaptic plasticity mechanisms that underlie some forms of learning and memory formation in the CNS. In the hippocampus and the neocortex, GluN1
Esther Del Cid-Pellitero et al.
Frontiers in systems neuroscience, 11, 17-17 (2017-04-15)
Neuronal activity is regulated in a homeostatic manner through changes in inhibitory GABA and excitatory glutamate (Glu) AMPA (A) receptors (GluARs). Using immunofluorescent staining, we examined whether calcium/calmodulin-dependent protein kinase IIα (CaMKIIα)-labeled (+) excitatory neurons in the barrel cortex undergo
Roshni Ramachandran et al.
Cephalalgia : an international journal of headache, 38(6), 1057-1070 (2017-07-26)
Background A common characteristic of migraine-inducing substances is that they cause headache and no pain in other areas of the body. Few studies have compared pain mechanisms in the trigeminal and spinal systems and, so far, no major differences have
Wen Fong Ooi et al.
PLoS genetics, 9(9), e1003795-e1003795 (2013-09-27)
Burkholderia pseudomallei (Bp), the causative agent of the often-deadly infectious disease melioidosis, contains one of the largest prokaryotic genomes sequenced to date, at 7.2 Mb with two large circular chromosomes (1 and 2). To comprehensively delineate the Bp transcriptome, we
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