C0733
Cyclooxygenase 1 from sheep
glycerol solution, ≥1500 units/mg protein
Sinónimos:
COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase
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About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32
Productos recomendados
form
glycerol solution
Quality Level
specific activity
≥1500 units/mg protein
mol wt
dimer subunit mol wt 70 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
sheep ... COX1(808251)
General description
Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain. Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.
Application
Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples and in prostaglandin synthase activity assay.
Biochem/physiol Actions
COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent KM of 8.3 μM for arachidonic acid.
Unit Definition
One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.
Physical form
Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN® 20, 300 μM diethyldithiocarbamate and 10% glycerol.
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Sze Chern Lim et al.
American journal of human genetics, 94(2), 209-222 (2014-01-28)
Leigh syndrome (LS) is a severe neurodegenerative disorder with characteristic bilateral lesions, typically in the brainstem and basal ganglia. It usually presents in infancy and is genetically heterogeneous, but most individuals with mitochondrial complex IV (or cytochrome c oxidase) deficiency
COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: cloning, structure, and expression
Chandrasekharan NV, et al.
Proceedings of the National Academy of Sciences of the USA, 99(21), 13926-13931 (2002)
Michela Zago et al.
Toxicology letters, 226(2), 107-116 (2014-01-30)
Diseases due to cigarette smoke exposure, including chronic obstructive pulmonary disease (COPD) and lung cancer, are associated with chronic inflammation typified by the increased expression of cyclooxygenase-2 (COX-2) protein. RelB is an NF-κB family member that suppresses cigarette smoke induction
Expression and activity of COX-1 and 2 and 5-LOX in joint tissues from dogs with naturally occurring coxofemoral joint osteoarthritis
Lascelles BD, et al.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 27(9), 1204-1208 (2009)
The Sigma class glutathione transferase from the liver fluke Fasciola hepatica
LaCourse EJ, et al.
PLoS Neglected Tropical Diseases, 6(5), e1666-e1666 (2012)
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