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MilliporeSigma

C0712

Sigma-Aldrich

Sustrato quimioluminiscente CDP-Star®

0.25 mM in H2O

Sinónimos:

Disodio 2-cloro-5-(4-metoxispiro[1,2-dioxetano-3,2′-(5-clorotriciclo[3.3.1.13,7]decan])-4-il]-1-fenil fosfato

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About This Item

Fórmula empírica (notación de Hill):
C18H19Cl2O7Na2P
Número de CAS:
Peso molecular:
495.20
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

assay

≥75% (HPLC)

form

liquid

usage

sufficient for 20 blots detection of alkaline phosphatase-labeled molecules (10 × 10 cm)

concentration

0.25 mM in H2O

storage temp.

2-8°C

SMILES string

Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3

InChI

1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;;

InChI key

UKWLRLAKGMZXJC-VCCHNUGISA-L

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Application

CDP-Star® is a sensitive, chemiluminescent substrate for alkaline phosphatase that allows for the rapid, reproducible detection of alkaline phosphatase-labeled molecules in Northern, Southern, and Western blotting applications. Detection of alkaline phosphatase-labeled molecules with CDP-Star is extremely sensitive as a result of low background luminescence coupled with high intensity and prolonged light output from the enzyme catalysis. Following exposure to alkaline phosphatase-labeled molecules, maximum light emission occurs at approximately 60 minutes and continues for up to 24 hours, allowing for multiple film exposures and/or the sensitive detection of targets present in small amounts. For convenience, CDP-Star is supplied as a 0.25 mM, ready-to-use aqueous solution (i.e. no dilution is necessary). CDP-Star functions on both neutral and positively-charged nylon, giving the reagent added application versatility.

Features and Benefits

CDP-Star® is supplied as a 0.25 mM ready-to-use aqueous solution for use in a variety of membrane-based applications. CDP-Star functions on both neutrally-charged and positively-charged nylon, giving the reagent added application versatility.

Caution

Store at 2-8 °C protected from light.

Legal Information

CDP-Star® is a trademark of Tropix, Inc. Bedford, MA, USA and covered under U.S. Patent 5,326,882.
CDP-Star is a registered trademark of Tropix, Inc.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Protocolos

Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.

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