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BCK647-IV-IM-S

Sigma-Aldrich

In Vivo EdU Click Kit 647

sufficient for 100 assays

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.22

fluorescence

λex 641 nm; λem 663 nm

shipped in

wet ice

storage temp.

2-8°C

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General description

The Baseclick EdU In Vivo Kits provide a superior alternative to BrdU and [3H]thymidine assays for detection of cell proliferation in whole animals, showing no toxic effects. EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU In Vivo Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU In Vivo Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.The in vivo kits are designed in a sense that you combine one of the three baseclick EdU cell proliferation kits such as:

1. Imaging kit (BCK-IV-IM)
2. Flow cytometry kit (BCK-IV-FC)
3. High throughput screening – HTS kit (BCK-IV-HTS)

You can select the above kits with the dye of choice and the right EdU content for your animal model.

Depending on your animal model or the number of animals to be tested you can choose between three kit sizes S, M, and L with increasing EdU content, as indicated in the following table.

Kit Size Content of EdU
S 50 mg
M 500 mg
L 1000 mg

Application

in Vivo, Imaging

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificados de análisis (COA)

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Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

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