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A8678

Sigma-Aldrich

Agar

suitable for plant cell culture

Sinónimos:

Agar goma, Agar-agar

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About This Item

Fórmula lineal:
(C12H18O9)n
Número de CAS:
9002-18-0
EC Number:
232-658-1
MDL number:
MFCD00081288
UNSPSC Code:
50405901
NACRES:
NA.21

biological source

algae (Rhodophyceae)

form

powder

technique(s)

cell culture | plant: suitable

mp

80-90 °C (1.5% in water)

transition temp

transition temp 27-37 °C (1.5%)

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar or Agar-agar is one of the major components found in the cell wall of red algae. It is a polysaccharide complex, comprising about 70% agarose and 30% agaropectin.

Application

Washed agar has been used:
  • as a supplement (0.9% w/v) to Murashige and Skoog basal medium for plant growth
  • in the preparation of cocultivation medium for Agrobacterium tumefaciens mediated transformation in maize (Zea mays) hybrid Hi-II
  • as a component in the complete medium to regenerate protoplasts of Piriformospora indica
  • as a clean agar medium to culture Synechococcus WH7803 strain

Biochem/physiol Actions

Agar is an ester of sulfuric acid in a linear galactan form, that makes it soluble in hot water and insoluble in cold. Washed agar contain less impurities and low level of nutrients.

Preparation Note

Washed

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Fungal genetics and biology : FG & B, 46(8), 543-550 (2009-04-09)
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