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A4284

Sigma-Aldrich

L-Asparragina monohydrate

suitable for cell culture, BioReagent

Sinónimos:

4-Amida del ácido(S)-2-aminosuccínico, 4-Amida del ácido L-aspártico, Ácido (S)-(+)-2-aminosuccinámico

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About This Item

Fórmula lineal:
NH2COCH2CH(NH2)COOH · H2O
Número de CAS:
5794-13-8
Peso molecular:
150.13
Beilstein/REAXYS Number:
1723527
EC Number:
200-735-9
MDL number:
MFCD00151038
UNSPSC Code:
12352209
PubChem Substance ID:
24890843
NACRES:
NA.75

biological source

synthetic

product line

BioReagent

assay

≥99% (TLC)

form

powder

technique(s)

cell culture | mammalian: suitable

color

white to off-white

mp

233-235 °C (lit.)

SMILES string

[H]O[H].N[C@@H](CC(N)=O)C(O)=O

InChI

1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1

InChI key

RBMGJIZCEWRQES-DKWTVANSSA-N

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Application

L-Asparagine monohydrate has been used:
  • as a media supplement to culture Black Mexican Sweet (BMS) cells
  • to prepare Adams III media to supplement C+Y medium for culturing Streptococcus pneumoniae
  • in an asparagine-rich diet to study the E0771 syngeneic breast tumor growth and survival in mice

Biochem/physiol Actions

L-Asparagine (L-Asp) is a nonessential amino acid involved in the synthesis of critical cellular proteins of human cells. It is synthesized from oxaloacetic acid. L-Asp is used as a growth supplement for tumor cells in in vitro conditions.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Handbook of Clinical Neurology, 1199-1218 null
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The New phytologist, 228(3), 973-988 (2021-01-08)
Plasma membrane (PM) intrinsic proteins (PIPs) are aquaporins facilitating the diffusion of water and small solutes. The functional importance of the PM organisation of PIPs in the interaction with other cellular structures is not completely understood. We performed a pull-down
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Journal of agricultural and food chemistry, 63(22), 5364-5372 (2015-05-15)
Pyrazines are specific Maillard reaction compounds known to contribute to the unique aroma of many products. Most studies concerning the generation of pyrazines in the Maillard reaction have focused on amino acids, while little information is available on the impact
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L-asparaginase is an effective anti-tumor agent for acute lymphoblastic leukemia. This work presents the development of an activity determination of L-ASNase preparations for pharmaceutical quality control purposes, in accordance with analytical Quality by Design principles. Critical method attributes, the absorbance

Protocolos

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

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