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Key Documents

A2289

Sigma-Aldrich

Albumin, Tetramethylrhodamine isothiocyanate bovine

lyophilized powder

Sinónimos:

TRITC-albumin

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.25

biological source

bovine

Quality Level

assay

≥90% (Lowry)

form

lyophilized powder

extent of labeling

≥0.5 mol TRITC per mol albumin

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

General description

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

Application

Albumin, Tetramethylrhodamine isothiocyanate has been used:
  • in photoinduced release assay of polyethylene beads
  • for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
  • in fluorescent correlation spectroscopy

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.

Preparation Note

Prepared from crystallized and lyophilized albumin (A4378).

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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