A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

• Sinónimos: Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt
• Fórmula empírica (notación de Hill): C₂₃H₃₅N₉O₄S · C₂H₄O₂
• Número de CAS: 96087-38-6
• Peso molecular: 593.70
• MDL number: MFCD00210085
• UNSPSC Code: 12352200
• PubChem Substance ID: 24890604
• NACRES: NA.52

Propiedades

• grade: for molecular biology
• Quality Level: 200
• assay: ≥95%
• form: film or powder
• solubility: H₂O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
• storage temp.: −20°C
• SMILES string: CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
• InChI: 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
• InChI key: FFBLNTOMOSLSQM-AYEYRVMASA-N

Descripción

General description

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Biochem/physiol Actions

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Caution

Light sensitive.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)