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MilliporeSigma

A0480

Sigma-Aldrich

Anti-Actin (plant) antibody, Mouse monoclonal

clone 10-B3 (MAbGPa), purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-Actin (plant) antibody produced in mouse

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

10-B3 (MAbGPa), monoclonal

mol wt

antigen ~45 kDa

species reactivity

plant

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using corn leaves extracts

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Arabidopsis thaliana ... ACT8(841347)

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General description

Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).
Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class.

Immunogen

ACT8 protein.

Application

Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in:
  • two-layer phos-tag polyacrylamide gel electrophoresis (PAGE)
  • immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).
  • immunoblotting

Biochem/physiol Actions

In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Paolo Longoni et al.
Plant physiology, 169(4), 2874-2883 (2015-10-07)
Light-harvesting complex II (LHCII) is a crucial component of the photosynthetic machinery, with central roles in light capture and acclimation to changing light. The association of an LHCII trimer with PSI in the PSI-LHCII supercomplex is strictly dependent on LHCII
Mo Jia et al.
BMC plant biology, 13, 60-60 (2013-04-17)
The opaque2 mutant is valuable for producing maize varieties with enhanced nutritional value. However, the exact mechanisms by which it improves protein quality and creates a soft endosperm texture are unclear. Given the importance of improving nutritional quality in grain
David Seung et al.
The Journal of biological chemistry, 291(39), 20718-20728 (2016-07-28)
Arabidopsis leaf chloroplasts typically contain five to seven semicrystalline starch granules. It is not understood how the synthesis of each granule is initiated or how starch granule number is determined within each chloroplast. An Arabidopsis mutant lacking the glucosyl-transferase, STARCH
Soluble and filamentous proteins in Arabidopsis sieve elements
Batailler B, et al.
Plant Cell, 35(7), 1258-1273 (2012)
One Plant Actin Isovariant, ACT7, Is Induced by Auxin and Required for Normal Callus Formation
Kandasamy MK, et al.
Plant Cell, 13(7), 1541-1541 (2001)

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