68528
Peroxidase from Bjerkandera adusta
≥2.8 U/mg
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About This Item
biological source
fungus (Bjerkandera adusta)
Quality Level
form
powder
specific activity
≥2.8 U/mg
storage temp.
−20°C
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
Application
Peroxidase, product 68528, is isolated from Bjerkandera adusta. Peroxidases from B. adusta, a white rot fungus, is used to degrade synthetic dyes . Peroxidase is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Peroxidases are used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Peroxidase is commonly used to determine amounts of glucose and peroxides in solution.
Biochem/physiol Actions
The transformation of industrial dyes by manganese peroxidases from Bjerkandera adusta is a manganese-independent reaction .
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Unit Definition
1 U corresponds to the amount of enzyme which oxidizes 1 μmol Mn2+ to Mn3+ per minute at pH 4.5 and 25°C (in the presence of H2O2).
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
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A Heinfling et al.
Applied and environmental microbiology, 64(8), 2788-2793 (1998-08-04)
We investigated the transformation of six industrial azo and phthalocyanine dyes by ligninolytic peroxidases from Bjerkandera adusta and other white rot fungi. The dyes were not oxidized or were oxidized very little by Phanerochaete chrysosporium manganese peroxidase (MnP) or by
G M Cook et al.
Microbiology (Reading, England), 144 ( Pt 12), 3297-3308 (1999-01-12)
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