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67455

Sigma-Aldrich

Atto 514 NHS ester

suitable for fluorescence

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

assay

90% (Coupling efficiency with benzylamine, HPLC)

form

solid

mol wt

Mw 1111 g/mol

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
514 nm

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

λ

(water/methanol with 0.1% perchloric acid)

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield and exceptional thermal and photo-stability. Thus Atto 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is the 514 nm line of the Argon-Ion laser.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Hsin-Yi Hsieh et al.
ACS nano, 13(4), 4486-4495 (2019-03-12)
Interest in developing a rapid and robust DNA sequencing platform has surged over the past decade. Various next-/third-generation sequencing mechanisms have been employed to replace the traditional Sanger sequencing method. In sequencing by synthesis, a signal is monitored by a

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