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47614

Sigma-Aldrich

Fluorescamine

BioReagent, suitable for fluorescence, ≥99.0% (UV)

Sinónimos:

Fluorescamine, 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

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About This Item

Fórmula empírica (notación de Hill):
C17H10O4
Número de CAS:
38183-12-9
Peso molecular:
278.26
Beilstein/REAXYS Number:
921143
EC Number:
253-814-5
MDL number:
MFCD00005928
UNSPSC Code:
12352200
PubChem Substance ID:
329757329

product line

BioReagent

assay

≥99.0% (UV)

mp

153-157 °C (lit.)
153-157 °C

solubility

acetonitrile: soluble
ethanol: soluble

fluorescence

λex 234 nm
λex 390 nm; λem 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)

suitability

suitable for fluorescence

SMILES string

O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14

InChI

1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H

InChI key

ZFKJVJIDPQDDFY-UHFFFAOYSA-N

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Application

Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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