45125
Elastase from porcine pancreas
lyophilized, powder, white, ~8 U/mg
Sinónimos:
Elastase from hog pancreas, Pancreatopeptidase E
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About This Item
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
12352204
Productos recomendados
biological source
Porcine pancreas
Quality Level
form
powder
quality
lyophilized
specific activity
~8 U/mg
mol wt
Mr ~25000
impurities
salt, none detected
color
white
storage temp.
−20°C
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Biochem/physiol Actions
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol 4-nitroaniline per minute at 25°C and pH 7.8 (succinyl-(L-Ala)3-4-nitroanilide as substrate)
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Other Notes
For protein sequence studies, limited proteolysis; Application in (selective) hydrolysis/condensation of carboxylic ester bonds
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I Grunnet et al.
The Biochemical journal, 209(1), 215-222 (1983-01-01)
Fatty acid synthetase from goat mammary gland was subjected to limited proteolysis by trypsin and elastase. Both proteolytic enzymes selectively cleaved the chain-terminating thioester hydrolase component from the enzyme complex, leaving all other partial activities intact in the core peptides.
T. Sakurai et al.
Journal of the American Chemical Society, 110, 7236-7236 (1988)
Commentary on 'Performance of a modified rabbit model of abdominal aortic aneurysm induced by topical application of porcine elastase: 5-month follow-up study'.
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Measurement of exhaled breath condensate (EBC) biomarkers offers a noninvasive means to assess airway disease, but the ability of EBC biomarkers to track longitudinal changes in disease severity remains unproven. EBC was collected from pediatric patients with cystic fibrosis (CF)
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A single instillation of porcine pancreatic elastase (PPE) results in significant airspace enlargement on the 28th day after instillation, whereas cigarette smoke (CS) exposure requires 6 months to produce mild emphysema in rodents. Considering that there are differences in the
Chromatograms
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