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44670

Sigma-Aldrich

Fast Blue BB Salt hemi(zinc chloride) salt

for microscopy (Hist.)

Sinónimos:

4-Amino-2,5-diethoxybenzanilide diazotated zinc double salt, 4-Benzoylamino-2,5-diethoxybenzenediazonium chloride hemi(zinc chloride) salt

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About This Item

Fórmula lineal:
C17H18N3O3Cl · 1/2 ZnCl2
Número de CAS:
5486-84-0
Peso molecular:
415.94
Colour Index Number:
37175
Beilstein/REAXYS Number:
1845288
EC Number:
226-817-4
MDL number:
MFCD00074765
UNSPSC Code:
12171500
PubChem Substance ID:
57650235
NACRES:
NA.47

grade

for microscopy (Hist.)

Quality Level

100

form

powder

mp

157 °C

solubility

hot water: 0.01 g/10 mL

εmax

≥100 at 390-400 nm in water
≥200 at 325-335 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CCOc1cc([N+]#N)c(OCC)cc1NC(=O)c2ccccc2.CCOc3cc([N+]#N)c(OCC)cc3NC(=O)c4ccccc4

InChI

1S/2C17H17N3O3.4ClH.Zn/c2*1-3-22-15-11-14(20-18)16(23-4-2)10-13(15)19-17(21)12-8-6-5-7-9-12;;;;;/h2*5-11H,3-4H2,1-2H3;4*1H;/q;;;;;;+2/p-2

InChI key

CMFRFQODFZBKTI-UHFFFAOYSA-L

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General description

Fast blue BB salt hemi(zinc chloride) salt is suitable as a common coloring agent for TLC (thin layer chromatography) analysis of cannabis. It is also suitable for histochemistry studies. It is of 415.9kDa.

Application

Fast blue BB salt hemi(zinc chloride) salt has been used to prepare substrate solution for tissue-blot enzyme-immunoassay procedure. It has been used in immunohistochemical staining.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Marjorie B Medina
Journal of agricultural and food chemistry, 59(5), 1565-1571 (2011-02-12)
A new method for the detection of phenolics in food systems was developed. This method is based on interactions of phenolics with Fast Blue BB diazonium salt in alkali pH, forming azo complexes, with the absorbance measured at 420 nm
B Algermissen et al.
Experimental dermatology, 3(6), 290-297 (1994-12-01)
In order to gain insights into the dynamics of mast cell subpopulations in normal and diseased skin, a novel enzyme-histochemical double and triple staining method was employed that allowed the detection of metachromasia (toluidine blue) and the mast cell proteases
H A Van Lith et al.
Electrophoresis, 12(12), 1045-1050 (1991-12-01)
There is no specific assay for rat plasma esterase-1 (ES-1) activity. Plasma contains many esterases, while known substrates do not discriminate between esterases. With gel electrophoresis, plasma esterase isozymes can be separated. Thus, a method consisting of gradient polyacrylamide gel
T Fujimoto et al.
Histochemistry, 65(3), 217-222 (1980-01-01)
The correlated distribution of intramembranous particles and sulfhydryl groups was examined in normal adult rabbit erythrocyte ghosts. Ghosts were treated to exhibit characteristic patterns of particle distribution and sulfhydryl groups were stained with Fast Blue BBN. It was shown that
R B Hughes et al.
Journal of forensic sciences, 24(4), 842-846 (1979-10-01)
We are reporting what we believe to be a safe, rapid TLC system and spray for the identification of marihuana. The use of Fast Blue 2B salt greatly enhances the specificity of the TLC analysis of suspected marihuana samples.
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