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Documentos clave

11012007

SK-GT-4 cell line human

Sinónimos:

SKGT4 Cells

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About This Item

UNSPSC Code:
41106514

biological source

human esophagus

form

liquid

packaging

tube of 5 μg 11012007-DNA-5UG
pkg of vial of cells 11012007-1VL

growth mode

Adherent

karyotype

Modal number 59 chromosomal aberration at 11p13-15.

morphology

Epithelial growing in islands

products

Expression of actin imtermediate filaments and growth factors TGF?1, TGF?2, TGF?3, TGF? and platelet-derived growth factor A (PDGFA). P53 mutation c.298C>T

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

Cell Line Origin

Oesophagus adenocarcinoma, well-differentiated

Application

For complete product information, please see ECACC
Recommended culture medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Culture Medium

RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cell population doubling time 39 hours.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections supplied by us, are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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E Rodriguez et al.
Cancer research, 50(19), 6410-6416 (1990-10-01)
In a cytogenetic analysis of 9 gastric and lower esophageal adenocarcinomas, we detected nonrandom rearrangements involving the region 11p13-15 in 8, thus identifying for the first time a specific chromosomal lesion in these tumors. In addition, rearrangements involving 3p21, translocations
N Altorki et al.
Cancer, 72(3), 649-657 (1993-08-01)
Gastric carcinoma is one of the most common malignancies worldwide, with an overall survival of about 10%. Improvement in therapy awaits better understanding of the biologic behavior of this tumor. Establishment of cell lines permits detailed analysis of the biology
G K Schwartz et al.
Journal of the National Cancer Institute, 85(5), 402-407 (1993-03-03)
Gastric adenocarcinoma is a common neoplasm worldwide. Patients with completely resected disease often have locoregional recurrence, and adjuvant chemotherapy has failed to reduce the common occurrence of metastases. Protein kinase C (PKC) is thought to be important in tumor cell
Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the

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