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MilliporeSigma

P5631

Sigma-Aldrich

o-Cresolphthalein Complexone

powder

Sinónimos:

o-Cresolphthalein-3′,3′′-bis-methyleneiminodiacetic acid, o-Cresolphthalexon, Metal phthalein, Phthalein purple, Xylenylphthalein-bisiminodiacetic acid

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About This Item

Fórmula empírica (notación de Hill):
C32H32N2O12
Número de CAS:
Peso molecular:
636.60
Beilstein/REAXYS Number:
381994
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

technique(s)

titration: suitable

color

white to off-white

mp

181-185 °C (dec.) (lit.)

solubility

methanol: 1 mg/mL

suitability

suitable for determination of calcium

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(cc(CN(CC(O)=O)CC(O)=O)c1O)C2(OC(=O)c3ccccc23)c4cc(C)c(O)c(CN(CC(O)=O)CC(O)=O)c4

InChI

1S/C32H32N2O12/c1-17-7-21(9-19(29(17)43)11-33(13-25(35)36)14-26(37)38)32(24-6-4-3-5-23(24)31(45)46-32)22-8-18(2)30(44)20(10-22)12-34(15-27(39)40)16-28(41)42/h3-10,43-44H,11-16H2,1-2H3,(H,35,36)(H,37,38)(H,39,40)(H,41,42)

InChI key

IYZPEGVSBUNMBE-UHFFFAOYSA-N

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General description

o-Cresolphthalein Complexone is a pH indicator dye commonly used for the calorimetric determination of calcium in serum.

Application

o-Cresolphthalein Complexone has been used in the estimation of calcium in serum samples based on the reaction of Ca2+ with o-cresolphthalein complexone in an alkaline solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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J Toffaletti et al.
Clinical chemistry, 26(11), 1562-1565 (1980-10-01)
We adapted a method for dialyzable calcium from fluorometric detection by use of calcein to a more specific spectrophotometric determination with cresolphthalein complexone. The reagents are available commercially and perform satisfactorily with respect to noise level, drift, stability, and sensitivity.
Improved determination of serum calcium with 2-cresolphthalein complexone.
K Lorentz
Clinica chimica acta; international journal of clinical chemistry, 126(3), 327-334 (1982-12-23)
Improved linearity of the calcium-cresolphthalein complexone reaction with sodium acetate.
D M Cowley et al.
Clinical chemistry, 32(5), 894-895 (1986-05-01)
C M Corns et al.
Annals of clinical biochemistry, 24 ( Pt 4), 345-351 (1987-07-01)
The reasons for the non-linearity of the standard curve for the calcium-cresolphthalein complexone reaction have been investigated. Calcium forms both 1:1 and 2:1 complexes with cresolphthalein complexone; at low calcium concentrations the 1:1 complex predominates and causes non-linearity. At high
Sandra F Williams et al.
Mayo Clinic proceedings, 80(12), 1655-1657 (2005-12-14)
Severe hypocalcemia may require prompt intervention to avoid life-threatening consequences. We report a case in which a 78-year-old man had a critically low serum calcium level measured with use of standard colorimetric assay after gadodiamide administration during magnetic resonance angiography.

Protocolos

Suitability for the Qualitative Determination of Calcium with Phthalein Purple (O-Cresophthalein Complexone) using Stannous Chloride

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