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MilliporeSigma

D9015

Sigma-Aldrich

3,3′-Diaminobencidina tetrahydrochloride hydrate

≥96% purity (TLC), powder

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About This Item

Fórmula lineal:
C12H14N4 · 4HCl · xH2O
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

3,3′-Diaminobencidina tetrahydrochloride hydrate, ISOPAC®

Quality Level

assay

≥96% (TLC)

form

powder

color

off-white to faint red, to beige

mp

280 °C

solubility

water: 50 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

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Application

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

Packaging

Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.

Caution

Injecting a compatible solvent permits preparation of any desired strength solution without exposure.

Reconstitution

Dissolving the contents in 100 mL of solvent yields a 0.1% solution.

Legal Information

Isopac is a registered trademark of Merck KGaA, Darmstadt, Germany

ISOPAC of

Referencia del producto
Descripción
Precios

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Artículos

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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