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D8284

Sigma-Aldrich

Bicinchoninic acid disodium salt hydrate

≥98% purity (HPLC), powder

Sinónimos:

2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt, 4,4′-Dicarboxy-2,2′-biquinoline disodium salt, BCA, Disodium 2,2′-biquinoline-4,4′-dicarboxylate

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About This Item

Fórmula empírica (notación de Hill):
C20H10N2Na2O4 · xH2O
Número de CAS:
979-88-4
Peso molecular:
388.28 (anhydrous basis)
MDL number:
MFCD10567219
UNSPSC Code:
12171500
PubChem Substance ID:
24894202
NACRES:
NA.47

product name

Bicinchoninic acid disodium salt hydrate, ≥98% (HPLC)

Quality Level

200

assay

≥98% (HPLC)

form

powder

color

white to light yellow

solubility

0.5% sodium bicarbonate: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

O.[Na+].[Na+].[O-]C(=O)c1cc(nc2ccccc12)-c3cc(C([O-])=O)c4ccccc4n3

InChI

1S/C20H12N2O4.2Na.H2O/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;;/h1-10H,(H,23,24)(H,25,26);;;1H2/q;2*+1;/p-2

InChI key

FAFVGCAYKZKOPT-UHFFFAOYSA-L

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Application

  • Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
  • It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
  • It has been used for determining protein concentration.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
SLCH3254
SLCF6008
SLCB6564
SLBX3261
SLBV1351

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Detection and characterization of chitinases and other chitin-modifying enzymes.
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Journal of Industrial Microbiology & Biotechnology, 30, 627-635 (2003)
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