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Key Documents

75482

Sigma-Aldrich

Gram′s decolorizer solution

for Gram staining

Sinónimos:

Acetone ethanol mixture

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About This Item

Número de CAS:
37348-17-7
MDL number:
MFCD00240361
UNSPSC Code:
12171500
NACRES:
MA.02

Quality Level

100

form

liquid

technique(s)

microbe id | staining: suitable

refractive index

n20/D 1.361

density

0.80 g/mL at 20 °C

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

General description

Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolorizer solution is a mixture of ethanol and acetone.

Application

Gram′s decolorizer solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolorizer dissolves the lipid layer from the gram-negative cells.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Central nervous system

supp_hazards

EUH066

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

68.0 °F

flash_point_c

20 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
BCCG4116
BCCD7425
BCCC5370
BCCB6462
BCBZ9634

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On the Trail of Campylobacter

On the Trail of Campylobacter - The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis. However, other species, like C. fetus, which causes spontaneous abortions, have also been associated with human illness. Campylobacter are Gram-negative, spiral-shaped, microaerophilic and motile bacteria with uni- or bi-polar fl agella

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

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