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MilliporeSigma

67432

Millipore

Campylobacter Millichrome plus Supplement

pkg of 5 L, suitable for microbiology

Sinónimos:

Campylobacter chromogenic Supplement

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About This Item

UNSPSC Code:
41171614
NACRES:
NA.74

sterility

sterile; 0.45 μm filtered

Quality Level

form

dry powder

shelf life

limited shelf life, expiry date on the label

packaging

pkg of 5 L

technique(s)

microbiological culture: suitable

solubility

sterile water: 21 mg/mL

application(s)

food and beverages
microbiology

shipped in

ambient

storage temp.

2-8°C

suitability

selective for bacteria: Campylobacter spp.

General description

Campylobacter Millichrome plus supplement is a selective media supplement for the detection of thermotolerant Campylobacter. The supplement provides additional selectivity to the Campylobacter Millichrome plus Agar Base. Millichrome chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 1 vial of supplement sufficient for 5 liters of final media. The supplement must be added to the melted Campylobacter Millichrome plus Agar Base at 45-50 °C.

Application

Campylobacter Millichrome plus supplement is a selective chromogenic culture media supplement intended for use in the qualitative direct detection, differentiation and presumptive identification of pathogenic biotypes of thermotolerant Campylobacter from various samples

Components

Composition in g/l:
(1 vial is sufficient for 5 L of final media)
Chromogenic and selective mix 1.05 g

Preparation Note

  • In a transparent vessel, add 210 mg of Campylobacter Millichrome plus Supplement to 10 ml purified water.
  • Swirl well until complete dissolution.
  • Filter to sterilize at 0.45 μm.
  • Add the 10 ml of the supplement solution to 1 liter of the melted Campylobacter Chromocult® plus Agar base at 45-50 °C.
  • Swirl or stir gently to homogenize.

Analysis Note

The Campylobacter colonies are colored red after 48 h of incubation at an optimal growth temperature of 42°C, whereas the growth of other species is inhibited or forms blue colonies after incubation at the same temperature.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Sol. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

4.1B - Flammable solid hazardous materials

wgk_germany

WGK 3


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