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MCD8MAG-48K

Millipore

MILLIPLEX® Mouse CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple cytokine and chemokine CD8+ biomarkers in mouse serum, plasma and cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

standard curve range: 12.2-50,000 pg/mL
(IL-6)

standard curve range: 2.4-10,000 pg/mL
(Granzyme B, IL-4, TNFα)

standard curve range: 24.4-100,000 pg/mL
(sFas, sFas Ligand, GM-CSF)

standard curve range: 3.7-15,000 pg/mL
(IL-5)

standard curve range: 4.9-20,000 pg/mL
(sCD137 IFNγ, IL-2, MIP-1ß, RANTES)

standard curve range: 48.8-200,000 pg/mL
(IL-13)

standard curve range: 6.1-25,000 pg/mL
(IL-10)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

CD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells.

The MILLIPLEX Mouse CD8+ T Cell Bead Panel enables you to focus on the therapeutic potential of Mouse CD8+ T Cell analytes. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of several analytes simultaneously, which can dramatically improve productivity.

The MILLIPLEX® Mouse CD8+ T Cell Bead Panel is the most versatile system available for Mouse CD8+ T Cell research.

This MILLIPLEX® kit offers you the ability to:
  • Select a 15-plex premixed option or
  • Choose any combination of analytes from our panel of 15 analytes to design a custom kit that better meets your needs.
  • A convenient “all-in-one” box format gives you the assurance that you will have all the necessary reagents you need to run your assay.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
There was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol)..

Application

  • Analytes: sCD137, sFas, sFAS ligand, GM-CSF, Granzyme B, IFNγ, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, MIP-1β, RANTES, TNF-α
  • Recommended Sample type: serum, plasma or tissue/cell lysate and culture supernatant
  • Recommended Sample dilution: neat
  • Assay Run Time: One day or overnight
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

6538 is a trademark of American Type Culture Collection
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids


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Virus-induced CD8+ T cells accelerate the onset of experimental autoimmune encephalomyelitis: implications for how viral infections might trigger multiple sclerosis exacerbations.
Rainey-Barger, EK; Blakely, PK; Huber, AK; Segal, BM; Irani, DN
Journal of Neuroimmunology null
Shimin Wang et al.
Scientific reports, 6, 24249-24249 (2016-04-15)
Regulatory T cells (Tregs) expand in peripheral lymphoid organs and can produce immunosuppressive cytokines to support tumor growth. IL-10 abrogation efficiently induces Treg formation but dampens tumoral neuropilin-1 (Nrp-1) Treg signaling, which simultaneously augments Th1 and Th17 immunity. These effects
Delphine Le Guennec et al.
Cancers, 14(1) (2022-01-12)
Our goal was to evaluate the effect of spontaneous physical activity on tumour immunity during aging. Elderly (n = 10/group, 33 weeks) ovariectomized C57BL/6J mice fed a hyperlipidic diet were housed in standard (SE) or enriched (EE) environments. After 4

Contenido relacionado

See how multiplexing the inflammation signaling pathway with MILLIPLEX® inflammation assays or cell signaling assays can help researchers bridge the gap between immunology and cell signaling, including investigating T cell signaling, Th Cell differentiation, inflammatory response signaling, and sepsis signaling.

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