Saltar al contenido
MilliporeSigma
Todas las fotos(7)

Key Documents

MABT880

Sigma-Aldrich

Anti-SUN2 Antibody, clone 3.1E

clone 3.1E, from mouse

Sinónimos:

SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

3.1E, monoclonal

species reactivity

human, rat, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... SUN2(25777)

General description

SUN domain-containing protein 2 (UniProt Q9UH99; also known as Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2) is encoded by the SUN2 (also known as FRIGG, KIAA0668, RAB5IP, UNC84B) gene (Gene ID 25777) in human. It is a single-pass nuclear envelope transmembrane protein that is highly expressed in heart, lung, and muscle. It has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. It may also facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage. It anchors chromosome movement in the prophase of meiosis and is required for telomere attachment to nuclear envelope and gametogenesis. SUN2 protein may also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5. Slight overexpression of SUN2 protein is seen in heart tissues form patients with congenital heart defects.

Specificity

Target specificity of clone 3.1E was verified by immunocytochemistry and Western blotting analyses using fibroblasts and heart tissue samples from Sun2-knockout mice.

Immunogen

GST-tagged recombinant human SUN2 N-terminal LMNA-binding region fragment.

Application

Research Category
Cell Structure
This mouse monoclonal Anti-SUN2 Antibody, clone 3.1E, Cat. No. MABT880, is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN2.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SUN2 in 10 µg of HepG2 cell lysate.

Immunocytochemistry Analysis: A representative lot immunostained nucleus of fibroblasts from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Western Blotting Analysis: A representative lot detected SUN2 in heart tissue lysate from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot detected mouse testis tissue (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot tested on different mouse tissues (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot detected immortalized fibroblasts derived from SUN2 +/+, SUN2 -/- , Sun1 -/- double knockout Sun2 -/- Sun1 -/- mouse pups (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN2 in 10 µg of HeLa cell lysate.

Target description

~80 kDa observed. 80.31/82.50/79.61 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Andres Ramirez-Martinez et al.
Nature communications, 12(1), 690-690 (2021-01-31)
Lamins and transmembrane proteins within the nuclear envelope regulate nuclear structure and chromatin organization. Nuclear envelope transmembrane protein 39 (Net39) is a muscle nuclear envelope protein whose functions in vivo have not been explored. We show that mice lacking Net39 succumb

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico