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Key Documents

MABD398

Sigma-Aldrich

Anti-DAX-1, clone 2F4 Antibody

ascites fluid, clone 2F4, from mouse

Sinónimos:

Dax1, Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1, AHC

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2F4, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_000466

UniProt accession no.

P51843

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NR0B1(190)

General description

DAX-1 is an orphan nuclear receptor and is required for the normal development of the hypothalamic-pituitary-adrenal-gonadal axis formation. Defects in DAX-1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC); also known as X-linked Addison disease (AHX). DAX-1 also has a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency by acting as a negative regulator of Oct3/4 in ES cells.

Immunogen

Linear peptide corresponding to Human DAX-1 (amino acids 135-166).

Application

Anti-DAX-1, clone 2F4, has been shown to work in Immunocytochemical (Lehmann et al., 2009) and Immunohistochemical (Ehrlund et al., 2013) analysis.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-DAX-1, clone 2F4 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of DAX-1.

Quality

Evaluated by Western Blot in a Dax-1 overexpressing HEK293 transfected cell line. Western Blot Analysis: A 1:1000 dilution of this antibody detected DAX-1 in a DAX-1 overexpressing HEK293 transfected cell line.

Target description

~ 52 kDa observed

Linkage

Replaces: 04-445

Physical form

Mouse monoclonal IgG1κ Ascites with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Extracellular matrix and hormones modulate DAX-1 localization in the human fetal adrenal gland.
Battista, Marie-Claude, et al.
The Journal of clinical endocrinology and metabolism, 90, 5426-5431 (2005)
Luisa A Helguero et al.
Endocrinology, 147(7), 3249-3259 (2006-04-22)
In recent studies, we have found that DAX-1 (dosage-sensitive sex reversal/adrenal hypoplasia congenita critical region on the X chromosome) is expressed in the mouse mammary epithelial cell line HC11. In this study, we focused on the regulation of DAX-1 expression
K T Tamai et al.
Molecular endocrinology (Baltimore, Md.), 10(12), 1561-1569 (1996-12-01)
Mutations in the human DAX-1 gene lead to X-linked adrenal hypoplasia congenita and hypogonadotropic hypogonadism. DAX-1 has been proposed to play a role in steroidogenesis because it is highly expressed in adrenocortical and testicular Leydig cells and because loss-of-function mutations
Anna Ehrlund et al.
Molecular and cellular biology, 29(8), 2230-2242 (2009-02-25)
Genetic and experimental evidence points to a critical involvement of the atypical mammalian orphan receptor DAX-1 in reproductive development and steroidogenesis. Unlike conventional nuclear receptors, DAX-1 appears not to function as a DNA-bound transcription factor. Instead, it has acquired the

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