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Key Documents

MAB88916-C

Sigma-Aldrich

Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free

clone 3E3, from mouse

Sinónimos:

Fibronectin, FN, CIG, Cold-insoluble globulin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

3E3, monoclonal

species reactivity

human

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)

General description

Fibronectin (FN), or in earlier literature also known as Cold-insoluble globulin (CIG), and is encoded by the gene named FN1/FN is an extracellular matrix protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Fibronectin also plays an important role in bone development and bone structure formation. Additionally, when fibronectin binds the protein anastellin it forms a multiprotein complex termed superfibronectin that has been shown to inhibit tumor growth, angiogenesis and metastasis. Fibronectin protein can be found extracellularly as well as intracellularly. There are multiple isoforms produced by alternative splicing. Plasma fibronectin (a soluble dimeric form) is secreted by hepatocytes into the blood. Cellular fibronectin (dimeric or cross-linked multimeric forms), are made by fibroblasts, epithelial and other cell types, and is deposited as fibrils in the extracellular matrix.

Immunogen

Epitope: This antibody recognizes the cell attachment fragment of Fibronectin.
Purified human Fibronectin, which contains the Cell Attachment Fragment, from amnion liquid.

Application

Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free is validated for use in western blotting, IHC, ELISA, ICC & inhibition for the detection of Fibronectin.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of U-251 cell lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Fibronectin (Cell Attachment Fragment) in human placenta and liver tissues.

ELISA Analysis: A representative lot detected the conformation of Fibronectin adsorbed onto self-assembled monolayers (SAMs) of alkanethiols over a range of Fibroectin surface densities (Kesolowsky, B. G., et al. (2003). J Biomed Mater Res A. 66(2):247-259.).

Immuncytochemistry Analysis: A representative lot detected Fibronectin (Cell Attachment Fragment) in Extracellular matrix deposited by HUVECs (Orecchia, A., et al. (2003). J Cell Sci. 116(Pt 17):3479-3489.).

Inhibition Analysis: A representative lot blocked cell-adhesive epitopes of Fibronectin (Cell Attachment Fragment) (Miller, D. C., et al. (2005). J Biomed Mater Res A. 73(4):476-84.).

Inhibition Analysis: A representative lot blocked RGD-dependent cell adhesion (Tsang, T. M., et al. (2012). J Biol Chem. 287(20):16759-16767.).

Inhibition Analysis: A representative lot inhibited the cell-adhesive epitopes of Fibronectin (Miller, D. C., et al. (2005). J Biomed Mater Res. 73(4):476-484.)

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of HepG2 cell lysate.

Target description

~260 kDa observed

Physical form

0.01M Phosphate Buffer containing no preservative, pH 7.1
Format: Purified
Protein G Purified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Synthetic peptide with cell attachment activity of fibronectin.
Pierschbacher, M, et al.
Proceedings of the National Academy of Sciences of the USA, 80, 1224-1227 (1983)
Detachment of cells from culture substrate by soluble fibronectin peptides.
Hayman, E G, et al.
The Journal of cell biology, 100, 1948-1954 (1985)
M D Pierschbacher et al.
The Journal of biological chemistry, 257(16), 9593-9597 (1982-08-25)
The complete amino acid sequence of the cell attachment domain of human plasma fibronectin (Pierschbacher, M. D., Hayman, E. G., and Ruoslahti, E. (1981) Cell 26, 259-267) has been determined by automated sequential degradation of a peptic fragment comprising this
Arg-Gly-Asp: a versatile cell recognition signal.
Ruoslahti, E and Pierschbacher, M D
Cell, 44, 517-518 (1986)
Christian Woltersdorf et al.
Matrix biology : journal of the International Society for Matrix Biology, 63, 91-105 (2017-02-14)
Interactions of cells with supramolecular aggregates of the extracellular matrix (ECM) are mediated, in part, by cell surface receptors of the integrin family. These are important molecular components of cell surface-suprastructures regulating cellular activities in general. A subfamily of β1-integrins

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