MAB5240
Anti-Phosphacan Antibody
culture supernatant, Chemicon®
About This Item
Productos recomendados
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
monoclonal
species reactivity
chicken
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgM
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PTPRZ1(5803)
General description
Specificity
Immunogen
Application
Neuroscience
Growth Cones & Axon Guidance
Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.
Immunohistochemistry: 1:20 Works on cold methanol or paraformaldehyde fixed tissues. Works on paraffin embedded tissues. Chondrotinase treatment of tissue consists of treating mounted tissue section with a solution of PBS plus 0.5U/mL of Chondroitinase ABC for 30 minutes at room temperature prior to blocking and antibody staining.
Immunocytochemistry: not tested
ELISA: Excellent for core and monomer protein.
Optimal working dilutions must be determined by the end user.
Physical form
Storage and Stability
Legal Information
Disclaimer
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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