MAB2503
Anti-Elastin Antibody, clone 10B8
clone 10B8, Chemicon®, from mouse
Sinónimos:
Anti-ADCL1, Anti-SVAS, Anti-WBS, Anti-WS
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10B8, monoclonal
species reactivity
mouse, bovine, human, chicken
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ELN(2006)
Specificity
Recognizes human Elastin. The antibody is also reactive with elastin from chicken, and bovine and mouse. Antibody specificity has been determined by immunoblotting of purified chick tropoelastin and immunoprecipitation of tritium-labeled chick tropoelastin from extracts of chick aorta organ cultures.
Immunogen
Tropoelastin from 17-day old lathyric chick embryo aortae, extracted an purified according to the method of Rich and Foster (1982) Meth. Enzymol. 82: 665-673.
Application
Anti-Elastin Antibody, clone 10B8 detects level of Elastin & has been published & validated for use in ELISA, IF, IP & WB.
Immunoblotting
Immunofluorescence
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunofluorescence
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Target description
66 kDa
Physical form
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Skin
Skin
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Thomas J Kean et al.
Connective tissue research, 57(4), 319-333 (2016-04-30)
To determine the effect of reduced (5%) oxygen tension on chondrogenesis of auricular-derived chondrocytes. Currently, many cell and tissue culture experiments are performed at 20% oxygen with 5% carbon dioxide. Few cells in the body are subjected to this supra-physiological
Fumiaki Sato et al.
Clinical biochemistry, 39(7), 746-753 (2006-05-13)
It is known that elastin mRNA is transcribed from a single gene. The variety of tropoelastin isoforms results from multiple alternative splicing of the primary transcript. The purpose of this study was to investigate the characteristics of elastic fiber assembled
Elastin exhibits a distinctive temporal and spatial pattern of distribution in the developing chick limb in association with the establishment of the cartilaginous skeleton.
Hurle, J M, et al.
Journal of Cell Science, 107 ( Pt 9), 2623-2634 (1994)
Long-term stability and safety of transgenic cultured epidermal stem cells in gene therapy of junctional epidermolysis bullosa.
De Rosa, L; Carulli, S; Cocchiarella, F; Quaglino, D; Enzo, E; Franchini, E; Giannetti et al.
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Qirui Hu et al.
Matrix biology : journal of the International Society for Matrix Biology, 29(7), 621-628 (2010-07-06)
Heterozygous elastin gene mutations cause autosomal dominant cutis laxa associated with emphysema and aortic aneurysms. To investigate the molecular mechanisms leading to cutis laxa in vivo, we generated transgenic mice by pronuclear injection of minigenes encoding normal human tropoelastin (WT)
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