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MAB1393Z

Sigma-Aldrich

Anti-PECAM-1 Antibody, clone TLD-3A12, azide free

clone TLD-3A12, Chemicon®, from mouse

Sinónimos:

CD31, MAB1393

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

TLD-3A12, monoclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

suitability

not suitable for immunohistochemistry (Paraffin)

UniProt accession no.

Q3SWT0

shipped in

dry ice

target post-translational modification

unmodified

Specificity

Rat PECAM-1. Antibody gives positive reaction at high dilutions against recombinant rat PECAM-1, confirming specificity.

Immunogen

Activated, Lewis rat derived microglial cells.

Application

Anti-PECAM-1 Antibody, clone TLD-3A12, azide free detects level of PECAM-1 & has been published & validated for use in ELISA, FC, WB, IH.
Flow Cytometry: 1:50-1:100

Immunohistology: frozen sections (-20°C methanol fixation), not recommended for traditional formalin fixed tissues.

ELISA

Western Blotting: 1:500. MW = 130-140 kDa

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Physical form

Format: Purified
Purified immunoglobulin by affinity chromatography on Protein G. Liquid in phosphate buffered saline containing no preservatives.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: Activated microglial cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Characterization of microglia and macrophages in the central nervous system of rats: definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal CNS and in four models of parenchymal reaction.
Flaris, N A, et al.
Glia, 7, 34-40 (1993)
An interferon-inducible molecule on brain endothelium which controls lymphocyte adhesion mediated by integrins.
Male, D, et al.
Immunology, 84, 453-460 (1995)
D L Livant et al.
Cancer research, 60(2), 309-320 (2000-02-10)
Using naturally serum-free SU-ECM basement membranes as invasion substrates showed that plasma fibronectin was necessary to stimulate invasion by DU 145 human and metastatic MATLyLu (MLL) rat prostate carcinoma cells. This activity mapped to the PHSRN sequence, which induced invasion
Yingchao Shi et al.
PLoS biology, 18(5), e3000705-e3000705 (2020-05-14)
Modeling the processes of neuronal progenitor proliferation and differentiation to produce mature cortical neuron subtypes is essential for the study of human brain development and the search for potential cell therapies. We demonstrated a novel paradigm for the generation of

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